期刊文献+

《世界胃肠病学杂志:英文版》

作品数21634被引量26844H指数29
本刊主要报道和刊登国内外、特别是我国消化病学者具有创造性的、有较高学术水平的基础和临床研究论文、研究快报。查看详情>>
  • 主办单位百世登出版集团有限公司
  • 出版周期年48期
共找到21,634篇文章
< 1 2 250 >
每页显示 20 50 100
Expression of calreticulin is associated with infiltration of T-cells in stage ⅢB colon cancer 被引量:2
1
作者 Rui-Qing Peng, Ya Ding, Xing Zhang, Yi-Xin Zeng, Xiao- Shi Zhang, State Key Laboratory of Oncology in South China, Biotherapy Center, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Ying-Bo Chen, Zhi-Wei Zhou, State Key Laboratory of Oncology in South China, Department of Gastric and Pancreatic Oncology, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Rong Zhang, State Key Laboratory of Oncology in South China, Department of Endoscopy, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Xing-Juan Yu, Yi-Xin Zeng, State Key Laboratory of Oncology in South China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2010年第19期2428-2434,共7页
AIM: To investigate the correlation between expression of calreticulin and infiltration of lymphocytes in stage ⅢB colon cancer. METHODS: Sixty-eight pathologically-confirmed speci-mens were obtained from stage ⅢB (... AIM: To investigate the correlation between expression of calreticulin and infiltration of lymphocytes in stage ⅢB colon cancer. METHODS: Sixty-eight pathologically-confirmed speci-mens were obtained from stage ⅢB (T3N1M0) colon cancer patients who underwent radical resection between January 1999 and May 2002 at the Cancer Center of Sun Yat-Sen University, Guangzhou, China. Immuno-histochemical analysis was performed to show infiltration of lymphocytes and expression of calreticulin in colon cancer. Association between calreticulin expression, infiltration of lymphocytes, and 5-year survival rate of patients was assessed. RESULTS: The expression level of calreticulin was lower in cancer nest than in its adjacent normal epithelium since 61.8% (42/68) of the samples were stained with calreticulin in colon cancer. The expression of calreticulin in colon cancer was associated with the infi ltration of CD45RO+ cells rather than with that of CD3+ cells. In addition, the stronger expression of calreticulin and the higher infiltration of CD3+ and CD45RO+ cells in colon cancer were associated with the higher 5-year survival rate of patients. CONCLUSION: Expression of calreticulin is associated with infiltration of T-cells, which implies that a low expression level of molecular marker may represent a new mechanism underlying immune escape in colon cancer. 展开更多
关键词 CALRETICULIN Tumor-infiltrating lymphocyte Colon cancer Immune escape
在线阅读 下载PDF
Outcome of surgical treatment of intestinal perforation in typhoid fever
2
作者 Aziz Sümer zgür Kemik +6 位作者 Ahmet Cumhur Dülger Aydemir Olmez Ismail Hasirci Erol Kisli Vedat Bayrak Gulay Bulut etin Kotan 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2010年第33期4164-4168,共5页
AIM:To represent our clinical experience in the treatment of intestinal perforation arising from typhoid fever.METHODS:The records of 22 surgically-treated patients with typhoid intestinal perforation were evaluated r... AIM:To represent our clinical experience in the treatment of intestinal perforation arising from typhoid fever.METHODS:The records of 22 surgically-treated patients with typhoid intestinal perforation were evaluated retrospectively.RESULTS:There were 18 males and 4 females,mean age 37 years(range,8-64 years).Presenting symptoms were fever,abdominal pain,diarrhea or constipation.Sixteen cases were subjected to segmental resection and end-to-end anastomosis,while 3 cases received 2-layered primary repair following debridement,one case with multiple perforations received 2-layered primary repair and end ileostomy,one case received segmental resection and end-to-end anastomosis followed by an end ileostomy,and one case received segmental resection and end ileostomy with mucous fistula operation.Postoperative morbidity was seen in 5 cases and mortality was found in one case.CONCLUSION:Intestinal perforation resulting from Salmonella typhi is an important health problem in Eastern and Southeastern Turkey.In management of this illness,early and appropriate surgical intervention is vital. 展开更多
关键词 Intestinal perforation Typhoid fever Treat- ment
在线阅读 下载PDF
Is early limited surgery associated with a more benign disease course in Crohn's disease? 被引量:1
3
作者 Petra Anna Golovics Laszlo Lakatos +11 位作者 Attila Nagy Tunde Pandur Istvan Szita Mihaly Balogh Csaba Molnar Erzsebet Komaromi Barbara Dorottya Lovasz Michael Mandel Gabor Veres Lajos S Kiss Zsuzsanna Vegh Peter Laszlo Lakatos 《世界胃肠病学杂志:英文版》 SCIE CAS 2013年第43期7701-7710,共10页
AIM:To analyze the difference in disease course and need for surgery in patients with Crohn’s disease(CD).METHODS:Data of 506 patients with incident CD were analyzed(age at diagnosis:31.5±13.8 years).Both hospit... AIM:To analyze the difference in disease course and need for surgery in patients with Crohn’s disease(CD).METHODS:Data of 506 patients with incident CD were analyzed(age at diagnosis:31.5±13.8 years).Both hospital and outpatient records were collected prospectively with a complete clinical follow-up and comprehensively reviewed in the population-based Veszprem province database,which includes incident CD patients diagnosed between January 1,1977 and December 31,2008.Follow-up data were collected until December 31,2009.All patients included had at least 1year of follow-up available.Patients with indeterminate colitis at diagnosis were excluded from the analysis.RESULTS:Overall,73 patients(14.4%)required resective surgery within 1 year of diagnosis.Steroid exposure and need for biological therapy were lower in patients with early limited surgery(P<0.001 and P=0.09).In addition,surgery rates during follow-up in patients with and without early surgery differed significantly after matching on propensity scores(P<0.001,HR=0.23).The need for reoperation was also lower in patients with early limited resective surgery(P=0.038,HR=0.42)in a Kaplan-Meier and multivariate Cox regression(P=0.04)analysis.However,this advantage was not observed after matching on propensity scores(PLogrank=0.656,PBreslow=0.498).CONCLUSION:Long-term surgery rates and overall exposure to steroids and biological agents were lower in patients with early limited resective surgery,but reoperation rates did not differ. 展开更多
关键词 Crohn’s DISEASE EARLY SURGERY DISEASE COURSE DISEASE behavior Treatment strategy
在线阅读 下载PDF
Clinical application of microRNA in gastric cancer in Eastern Asian area 被引量:1
4
作者 Ming Gao Hao Yin Zhe-Wei Fei 《世界胃肠病学杂志:英文版》 SCIE CAS 2013年第13期2019-2027,共9页
Recent research has shown that microRNA (miRNA), which is involved in almost every step of gastric carcinogenesis, has broad prospective application in diagnosis and therapy of gastric carcinoma. Eastern Asia (South K... Recent research has shown that microRNA (miRNA), which is involved in almost every step of gastric carcinogenesis, has broad prospective application in diagnosis and therapy of gastric carcinoma. Eastern Asia (South Korea, Japan and China) has the highest incidence of gastric cancer in the world. There were 988 000 new cases of gastric cancer worldwide and 736 000 deaths in 2008. Approximately 60% of the cases of gastric cancer are found in East Asia (mainly China). We herein provide a brief review of the clinical applications of miRNA, which include the following aspects: (1) miRNA may serve as a potential new generation of tumor markers; (2) a complete miRNA expression profile is highly specific, can reflect the evolutionary lineage and differentiation of tumors, and be used to carry out diversity analysis; (3) detecting specific miRNA expression in peripheral blood will become a new method for diagnosis of gastric cancer; (4) miRNA can predict prognosis of gastric cancer; (5) miRNA has predictive value in determining chemotherapy and radiotherapy resistance; and (6) miRNA could be a type of innovative drug. Finally, we focus on assessing the value of miRNA from laboratory to clinical application and the challenges it faces in East Asia. 展开更多
关键词 MICRORNA PROGNOSIS Clinical application GASTRIC cancer EASTERN ASIA
在线阅读 下载PDF
Diagnostic value of endothelial markers and HHV-8 staining in gastrointestinal Kaposi sarcoma and its difference in endoscopic tumor staging
5
作者 Naoyoshi Nagata Toru Igari +8 位作者 Takuro Shimbo Katsunori Sekine Junichi Akiyama Yohei Hamada Hirohisa Yazaki Norio Ohmagari Katsuji Teruya Shinichi Oka Naomi Uemura 《世界胃肠病学杂志:英文版》 SCIE CAS 2013年第23期3608-3614,共7页
AIM: To clarify the diagnostic values of hematoxylin and eosin (HE), D2-40, CD31, CD34, and HHV-8 immunohistochemical (IHC) staining in gastrointestinal Kaposi's sarcoma (GI-KS) in relation to endoscopic tumor sta... AIM: To clarify the diagnostic values of hematoxylin and eosin (HE), D2-40, CD31, CD34, and HHV-8 immunohistochemical (IHC) staining in gastrointestinal Kaposi's sarcoma (GI-KS) in relation to endoscopic tumor staging. METHODS: Biopsy samples (n = 133) from 41 human immunodeficiency virus-infected patients were reviewed. GI-KS was defined as histologically negative for other GI diseases and as a positive clinical response to KS therapy. The receiver operating characteristic area under the curve (ROC-AUC) was compared in relation to lesion size, GI location, and macroscopic appearances on endoscopy. RESULTS: GI-KS was confirmed in 84 lesions (81.6%). Other endoscopic findings were polyps (n = 9), inflammation (n = 4), malignant lymphoma (n = 4), and condyloma (n = 2), which mimicked GI-KS on endoscopy. ROC-AUC of HE, D2-40, blood vessel markers, and HHV-8 showed results of 0.83, 0.89, 0.80, and 0.82, respectively. For IHC staining, the ROC-AUC of D2-40 was significantly higher (P < 0.05) than that of HE staining only. In the analysis of endoscopic appearance, the ROC-AUC of HE and IHC showed a tendency toward an increase in tumor staging (e.g. , small to large, patches, and polypoid to SMT appearance). D2-40 was significantly (P < 0.05) advantageous in the upper GI tract and for polypoid appearance compared with HE staining. CONCLUSION: The diagnostic value of endothelial markers and HHV-8 staining was found to be high, and its accuracy tended to increase with endoscopic tumor staging. D2-40 will be useful for complementing HE staining in the diagnosis of GI-KS, especially in the upper GI tract and for polypoid appearance. 展开更多
关键词 Gastrointestinal Kaposi’s SARCOMA HEMATOXYLIN and EOSIN CD31 CD34 D2-40 Human herpesvirus-8
在线阅读 下载PDF
Effect of hepatic glucose production on acute insulin resistance induced by lipid-infusion in awake rats 被引量:2
6
作者 LingLi Gang-YiYang 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2004年第21期3208-3211,共4页
AIM: To explore the influence of hepatic glucose production on acute insulin resistance induced by a lipid infusion in awake rats. METHODS: A hyperinsulinaemic-euglycaemic clamp was established in awake chronically ca... AIM: To explore the influence of hepatic glucose production on acute insulin resistance induced by a lipid infusion in awake rats. METHODS: A hyperinsulinaemic-euglycaemic clamp was established in awake chronically catheterized rats. Two groups of rats were studied either with a 4-h intraarterial infusion of lipid/heparin or saline. Insulin-mediated peripheral and hepatic glucose metabolism was assessed by hyperinsulinaemic-euglycaemic clamp combined with [3-^3H]-glucose infusion. RESULTS: During hyperinsulinaemic-euglycaemic clamp,there was a significant increase in plasma free fatty acid (FFA, from 741.9±50.6 to 2346.44±238.5μmol/L, P<0.01) in lipid-infused group. The glucose infusion rates (GIR) in the lipid infusion rats, compared to control rats, were significantly reduced (200-240 min average: lipid infusion; 12.64±1.5 vs control; 34.04±1.6 mg/kg.min, P<0.01), declining to - 35% of the corresponding control values during the last time of the clamp (240min: lipid infusion; 12.04±1.9 vs control; 34.74±1.7 mg/kg·min, P<0.0001). At the end of clamp study,the hepatic glucose production (HGP) in control rats was significantly suppressed (88%) from 19.04±4.5 (basal) to 2.34±0.9 mg/kg.min (P<0.01). The suppressive effect of insulin on HGP was significantly blunted in the lipid-infused rats (200-240 min: from 18.74±3.0 to 23.24±3.1 mg/kg.min (P<0.05). The rate of glucose disappearance (GRd) was a slight decrease in the lipid-infused rats compared with controls during the clamp.CONCLUSION: These data suggest that lipid infusion could induces suppression of hepatic glucose production, impairs the abilities of insulin to suppress lipolysis and mediate glucose utilization in peripheral tissue. Therefore, we conclude that lipid-infusion induces an acute insulin resistance in vivo. 展开更多
关键词 葡萄糖代谢 肝脏 抵抗作用 胰岛素 急性作用 脂质灌输 苏醒 老鼠 导管插入
在线阅读 下载PDF
Metastatic liver cancer: A rare case 被引量:2
7
作者 Bong-Wan Kim Hee-Jung Wang +2 位作者 In-Ho Jeong Sang-Ick Ahn Myoung-Wook Kim 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2005年第27期4281-4284,共4页
Hemangiopericytoma is a rare tumor especially when it rises in the peritoneal cavity. We present a case of a 60-year-old woman with an isolated recurrent hemangiopericytoma of the liver. The patient presented with a p... Hemangiopericytoma is a rare tumor especially when it rises in the peritoneal cavity. We present a case of a 60-year-old woman with an isolated recurrent hemangiopericytoma of the liver. The patient presented with a palpable right upper quadrant abdominal mass, which occurred 7 years after undergoing resection of a malignant hemangiopericytoma arising from the greater omentum. She had not followed up 6 mo after surgery. Various imaging studies showed a single large, well-capsulated liver tumor with central necrosis, accompanied by hypervascularity typical of a vascular tumor. Preoperative laboratory HBsAg and anti-HCV workup were both negative. Under the impression of recurrent malignant hemangiopericytoma, right trisegmentectomy was performed to completely resect the tumor. Pathological examination confirmed the diagnosis of recurrent hemangiopericytoma. Even though the incidence of the hemangiopericytoma is relatively low, malignant hemangiopericytoma has a tendency to recur frequently after a long-term disease-free interval. Also, the recurrent hemangiopericytoma is not easily detected early during follow-up until it becomes symptomatic because there are no specific tumor markers, and because of the diversity with regard to site of recurrence. The authors suggest that Positron Emission Tomogram (PET) may be a useful tool for the detection of recurrent hemangiopericytoma. We describe herein some characteristics and behaviors of malignant hemangiopericytoma, particularly after surgical resection. 展开更多
关键词 肝癌 病理机制 病例报告 血管外皮细胞瘤
在线阅读 下载PDF
Interleukin-10 and chronic liver disease 被引量:1
8
作者 Li-Juan Zhang Xiao-Zhong Wang 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2006年第11期1681-1685,共5页
Interleukin (IL)-10 is an important immunoregulatory cytokine produced by many cell populations. Numerous investigations suggest that IL-10 plays a major role in chronic liver diseases. IL-10 gene polymorphisms are po... Interleukin (IL)-10 is an important immunoregulatory cytokine produced by many cell populations. Numerous investigations suggest that IL-10 plays a major role in chronic liver diseases. IL-10 gene polymorphisms are possibly associated with liver disease susceptibility or severity. Recombinant human IL-10 has been produced and is currently tested in clinical trials. These trials may give new insights into the immunobiology of IL-10 and suggest that the IL-10/IL-10 receptor system may become a new therapeutic target. 展开更多
关键词 白细胞介素-10 慢性肝脏疾病 病理机制 治疗
在线阅读 下载PDF
Incidence and mortality of gastric cancer in China 被引量:65
9
作者 Ling Yang 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2006年第1期17-20,共4页
胃的癌症是在世界上的最经常的癌症之一;几乎三分之二个胃的癌症盒子和死亡在更少的发达区域发生。在中国,基于在1970年代和1990年代进行的二国家死亡调查,有在这个国家的胃的癌症的地域分布的一个明显的聚类,与死亡主要是在农村区... 胃的癌症是在世界上的最经常的癌症之一;几乎三分之二个胃的癌症盒子和死亡在更少的发达区域发生。在中国,基于在1970年代和1990年代进行的二国家死亡调查,有在这个国家的胃的癌症的地域分布的一个明显的聚类,与死亡主要是在农村区域定位了的高度,特别在 Gansu ,河南省, Hebei ,在中国的中间西方的部分的山西和 Shaanxi 省。尽管有到 1990 年代初的从 1970 年代的细微增加,在胃的癌症死亡的显著衰落被注意在几乎全部人口在在中国的最后十年期间。在经济改革二十年以前开始了以后,这些衰落大部分由于在社会经济的环境,生活方式,营养,教育和护理系统的戏剧的改进。不过,胃的癌症将当前仍然是重要癌症负担并且在中国是在癌症预防和控制策略的关键问题之一。在 2005,从胃的癌症的 30 万死亡和 40 万个新盒子将评价第三很普通的癌症,这被预言。为在中国的胃的癌症的预防和控制策略的必要包裹将集中于控制 Helicobacter pylori (H。pylori ) 感染,改善教育层次,倡导的健康食谱和反烟草竞选,寻找划算的早察觉,诊断和治疗为可医好的管理和辩解的照顾包括途径编程序。 展开更多
关键词 胃癌 中国 死亡率 病理机制
在线阅读 下载PDF
Interaction between enteric epithelial cells and Peyer's patch lymphocytes in response to Shigella lipopolysaccharide: Effect on nitric oxide and IL-6 release
10
作者 Jie Chen Chuen-Pei Ng +4 位作者 Dewi K Rowlands Peng-Hui Xu Jie-Ying Gao Yiu-Wa Chung Hsiao-Chang Chan 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2006年第24期3895-3900,共6页
瞄准:在氮的氧化物的版本上调查在伤寒上皮细胞和 Peyer 的补丁的淋巴细胞之间的相互作用的效果(没有) 并且响应志贺氏菌属脂肪的多糖(LPS ) 的 IL-6。方法:人的结肠的上皮细胞(Caco-2 ) 是有 Peyer 的补丁从的淋巴细胞的混合 cocultu... 瞄准:在氮的氧化物的版本上调查在伤寒上皮细胞和 Peyer 的补丁的淋巴细胞之间的相互作用的效果(没有) 并且响应志贺氏菌属脂肪的多糖(LPS ) 的 IL-6。方法:人的结肠的上皮细胞(Caco-2 ) 是有 Peyer 的补丁从的淋巴细胞的混合 cocultured 野类型(C57 老鼠) 并且可诱导没有 synthase 大美人老鼠,并且与志贺氏菌属 F2a-12 LPS 质问了。版本没有并且 mIL-6 被 Griess 比色测定和连接酶的免疫吸着剂试金(ELISA ) 分别地测量。结果:当 LPS 挑战不在时,不在 Caco-2 上皮细胞的培养基然而并非在 Peyer 的补丁的淋巴细胞被检测,并且 NO 版本在有从也的淋巴细胞的两 cocultures 是进一步起来调整的野类型或 i NOS 大美人老鼠,与一显著地,高水平与 i NOS 猛烈淋巴细胞在 coculture 观察了。在为 24-h 的志贺氏菌属 F2a-12 LPS 挑战以后,没有生产显著地从野类型的老鼠然而并非从 i NOS 猛烈老鼠与 Peyer 的补丁的淋巴细胞在独自一个的 Caco-2 和 coculture 被增加。LPS 被发现从淋巴细胞刺激 mIL-6 的版本,它被 coculture 与 Caco-2 上皮细胞压制。在从 i NOS 猛烈老鼠的淋巴细胞的导致 LPS 的 mIL-6 生产从野类型的老鼠比那显著地大。结论:Peyer 的补丁的淋巴细胞从伤寒维持没有生产的组成的基础水平上皮的房间 Caco-2。从 Peyer 的补丁的淋巴细胞的导致 LPS 的 mIL-6 版本被 cocultured 上皮细胞压制。当没有变化在在淋巴细胞从的没有生产是可检测的时野类型并且在 LPS 前后的 i NOS 猛烈老鼠质问,不从淋巴细胞看起来起一个禁止的作用在上皮响应 LPS 的没有版本和他们的自己的 mIL-6 版本。 展开更多
关键词 肠上皮细胞 囊依赖性淋巴细胞 志贺氏菌病 脂多糖
在线阅读 下载PDF
Sustained low diffusing capacity in hepatopulmonary syndrome after liver transplantation 被引量:2
11
作者 Graciela Martínez-Pallí Federico P Gómez +5 位作者 Joan A Barberà Miquel Navasa Josep Roca Robert Rodríguez-Roisin Felip Burgos Conchi Gistau 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2006年第36期5878-5883,共6页
AIM: To study the presence of sustained low diffusing capacity (DLCO) after liver transplantation (LT) in patients with hepatopulmonary syndrome (HPS).METHODS: Six patients with mild-to-severe HPS and 24 without HPS w... AIM: To study the presence of sustained low diffusing capacity (DLCO) after liver transplantation (LT) in patients with hepatopulmonary syndrome (HPS).METHODS: Six patients with mild-to-severe HPS and 24 without HPS who underwent LT were prospectively followed before and after LT at mid-term (median, 15 mo). HPS patients were also assessed at long-tem (median, 86 mo).RESULTS: Before LT, HPS patients showed lower PaO2 (71 ± 8 mmHg), higher AaPO2 (43 ± 10 mmHg) and lower DLCO (54% ± 9% predicted), due to a combination of moderate-to-severe ventilation-perfusion (VA/Q) imbalance, mild shunt and diffusion limitation, than non-HPS patients (94 ± 4 mmHg and 19 ± 3 mmHg, and 85% ± 3% predicted, respectively) (P < 0.05 each). Seven non-HPS patients had also reduced DLCO (70% ± 4% predicted).At mid- and long-term after LT, compared to pre-LT, HPS patients normalized PaO2 (91 ± 3 mmHg and 87 ± 5 mmHg), AaPO2 (14 ± 3 mmHg and 23 ± 5 mmHg) and all VA/Q descriptors (P < 0.05 each) without changes in DLCO (53% ± 8% and 56% ± 7% predicted, respectively). Post-LT DLCO in non-HPS patients with pre-LT low DLCO was unchanged (75% ± 6% predicted).CONCLUSION: While complete VA/Q resolution in HPS indicates a reversible functional disturbance, sustained low DLCO after LT also present in some non-HPS patients, points to persistence of sub-clinical liver-induced pulmonary vascular changes. 展开更多
关键词 容量 肝移植 一氧化碳 肺交换
在线阅读 下载PDF
Dynamic changes and mechanism of intestinal endotoxemia in partially hepatectomized rats 被引量:1
12
作者 Cui-Ping Xu Juan Liu +3 位作者 Jin-Chun Liu De-Wu Han Yang Zhang Yuan-Chang Zhao 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2007年第26期3592-3597,共6页
AIM: To explore the mechanism of intestinal endo- toxemia (IETM) formation and its changes in partially hepatectomized (PH) rats. METHODS: One-hundred and two adult male Wistar rats were randomly divided into three gr... AIM: To explore the mechanism of intestinal endo- toxemia (IETM) formation and its changes in partially hepatectomized (PH) rats. METHODS: One-hundred and two adult male Wistar rats were randomly divided into three groups: normal control (NC) group,partially hepatectomized (PH) group and a sham-operated (SO) group. To study the dynamic changes,rats were sacrificed before and at different time points after partial hepatectomy or the sham-operation ( 6 h,12 h,24 h,36 h,48 h,72 h,120 h and 168 h). NC group was used as 0h time point in observation,namely 0 h group. For each time point indicated,six rats were used in parallel. Endotoxin (ET) and diamine oxidase (DAO) levels were determined in serum using Limulus Lysate test with chromogenic substrate and spectrophotometry. Intestinal mucosa barrier was observed under opticcal or electron microscope. The number and functional state of Kupffer cells (KCs) in the remnant regenerating liver were measured by immunohistochemical staining. RESULTS: Serum ET levels significantly increased during 6-72 h period after PH compared with NC and SO groups,and there were two peak values at 12 and 48 h while serum DAO level significantly increased at 12 and 24 h. There was positive correlation (r = 0.757,P < 0.05) between the levels of DAO and ET dynamic changes. The optical examination showed neutrophil margination and superficial necrosis of the villi in the intestinal mucosa during 6-24 h period after PH. The penetrated electron microscope examination showed thatthe gaps between intestinal mucosa cells were increased and the Lanthanum (La) particles were observed among the intestinal mucosa cells during 6-48 h period. The numbers of KCs in the remnant regenerating liver were significantly increased during 24-168 h period after PH. However,the activation of KCs was predominantly observed at 48 h after PH. CONCLUSION: The mechanism of IETM in PH rats might be the injury of intestinal mucosa barrier and the decrease of the absolute number of KCs as well as the depression of functional 展开更多
关键词 肝切除手术 肠内毒素 肠黏膜 治疗
在线阅读 下载PDF
Qualitative and quantitative analyses of the bifidobacterial microbiota in the colonic mucosa of patients with colorectal cancer, diverticulitis and inflammatory bowel disease 被引量:6
13
作者 Miguel Gueimonde Arthur Ouwehand +2 位作者 Heikki Huhtinen Eeva Salminen Seppo Salminen 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2007年第29期3985-3989,共5页
AIM: To characterize the bifidobacterial microbiota of the colonic mucosa in patients with colon cancer, inflammatory bowel disease or diverticulitis. METHODS: A sample of the distal colonic mucosa was taken during su... AIM: To characterize the bifidobacterial microbiota of the colonic mucosa in patients with colon cancer, inflammatory bowel disease or diverticulitis. METHODS: A sample of the distal colonic mucosa was taken during surgery from a total of 34 patients, twenty-one with diagnosed colorectal cancer, nine with diverticulitis and four with inflammatory bowel disease, requiring surgery for their condition. Bacterial DNA was extracted from the resected mucosal samples and bifidobacterial mucosa-associated microbiota was qualitatively and quantitatively determined by means of qualitative and quantitative PCR. RESULTS: Bifidobacteria were found in 100% of the samples from patients with diverticulitis or IBD and a 76% of those suffering colon cancer. The species B. longum and B. bifidum were the most widely found, followed by B. animalis, B. catenulatum and B. adolescentis. B. breve, B. dentium and B. angulatum were not detected in any sample. A significantly higher occurrence of B. longum was observed in patients with diverticulitis than in those with colon cancer or IBD (100%, 62% and 75%, respectively, P < 0.05). Similar results were obtained for B. animalis (56%, 0% and 25%, P < 0.05), while B. adolescentis was only found in the mucosa from patients with colon cancer (5 out of 21, 24%). At the quantitative level, patients with colon cancer or IBD showed lower counts of total Bifidobacterium (4.94 and 5.91 vs 6.96 log Cells/sample, respectively, P < 0.05) and of the species B. longum (4.05 and 4.79 vs 6.76, P < 0.05) than those with diverticulitis. CONCLUSION: Aberrancies in mucosa associated microbiota are present in different intestinal diseases. This may indicate a role of the microbiota in the pathogenesis of these diseases. 展开更多
关键词 结肠直肠癌 憩室炎 炎性肠病 结肠粘膜 微生物群
在线阅读 下载PDF
Duration of treatment with 5-aminosalicylic acid compounds
14
作者 T Moshkovska JF Mayberry 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2007年第32期4310-4315,共6页
The development of 5-aminosalicylic acid (5-ASA) therapy as a life long treatment for ulcerative colitis is reviewed from its origins in the 1940s to the present day. The drug was designed to treat rheumatoid arthriti... The development of 5-aminosalicylic acid (5-ASA) therapy as a life long treatment for ulcerative colitis is reviewed from its origins in the 1940s to the present day. The drug was designed to treat rheumatoid arthritis,but was found helpful in the management of nine patients with ulcerative colitis. This discovery preceded the emergence of the clinical trial as a tool for assessing a new drug's efficacy; as a result it lacked scientific rigour and was selective in its presentation of results. Nevertheless it identified the future cornerstone of therapy in ulcerative colitis. In 1962,the first double blind controlled trial of sulphasalazine was conducted on 40 patients. Outcome measures were subjective and included symptoms and an assessment of the rectal mucosa. In 1973,the first two papers on the role of sulphasalazine in maintenance of remission were published. Both used placebo controls and had a stratified design. Outcomes were measured using "an intention to treat" approach. The British study of 64 patients used both subjective and objective criteria to assess outcomes. Patients on placebo had a relapse rate four times patients on active treatment and this founded the basis for a life long approach to therapy with 5-ASA compounds in ulcerative colitis. However,in 1985,a small "on demand" study of 32 patients suggested this approach might be as effective as continuous treatment. Some support for this view came from an Italian study which showed no benefit to continued treatment for those in remission for two years or more. The central problem these studies identify is that of adherence to treatment in the long-term. Few studies have considered patients' attitudes to continuous therapy and it is an area that needs further investigation. 展开更多
关键词 大肠炎 大肠溃疡 5氨基水杨酸混合物 治疗持续时间
在线阅读 下载PDF
CO liberated from CORM-2 modulates the inflammatory response in the liver of thermally injured mice 被引量:3
15
作者 Bing-Wei Sun Yan Sun Zhi-Wei Sun Xi Chen 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2008年第4期547-553,共7页
AIM:To explore the effects of CO-releasing molecules tricarbonyldichlororuthenium(Ⅱ)dimer,CORM-2-liberated CO on attenuation of inflammatory responses in liver of an experimental animal model of thermal injury and to... AIM:To explore the effects of CO-releasing molecules tricarbonyldichlororuthenium(Ⅱ)dimer,CORM-2-liberated CO on attenuation of inflammatory responses in liver of an experimental animal model of thermal injury and to investigate the associated potential mechanisms.METHODS:Thirty-six mice were assigned to three groups in three respective experiments.In each experiment,mice in sham group(n=4)received sham thermal injury,whereas mice in burn group(n=4) received a 15%of total body surface area(TBSA)full-thickness thermal injury,and mice in burn+CORM-2 group(n=4)received the same thermal injury with immediate administration of CORM-2(8 mg/kg,iv).Hepatic tissue sections were stained with hematoxylin and eosin and examined under a light icroscope.Levels of aminotransferases(ALT and AST)and nitric oxide(NO) were measured by biochemical methods.Tumor necrosis factor-α(TNF-α)and interleukin(IL-1β)activity,and the protein expression of iNOS and HO-1 in serum and tissue homogenates were assessed.In in vitro experiments,Kupffer cells were stimulated with LPS(10μg/mL)for 4 h in the presence or absence of CORM-2(10-100μmol/L).Subsequently,the expression levels of TNF-αand NO production were assessed.RESULTS:Pro-inflammatory mediators(TNF-α,IL-1β,NO)in serum and liver homogenates of thermally injured mice were significantly reduced by CORM-2 administration.This was accompanied by a decrease in the expression of iNOS while an increase in the expression of HO-1 in the liver tissue.In parallel,the concentrations of TNF-αand NO in supernatants of LPS-stimulated Kupffer cells co-incubated with CORM-2 (10-100μmol/L)were also markedly decreased.Histological examination demonstrated that CORM-2 could attenuate the leukocytes infiltration to the liver tissue. CONCLUSION:CORM-released CO modulates liver inflammation and significantly protects liver injury in burn mice by inhibiting the expression of iNOS and NO production,down-regulating the expression of pro-inflammatory mediators(TNF-α,IL-1β). 展开更多
关键词 一氧化碳 热伤 肝脏 炎症
在线阅读 下载PDF
Autophagy and ethanol-induced liver injury 被引量:5
16
作者 Terrence M Donohue Jr 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2009年第10期1178-1185,共8页
The majority of ethanol metabolism occurs in the liver. Consequently, this organ sustains the greatest damage from ethanol abuse. Ethanol consumption disturbs the delicate balance of protein homeostasis in the liver, ... The majority of ethanol metabolism occurs in the liver. Consequently, this organ sustains the greatest damage from ethanol abuse. Ethanol consumption disturbs the delicate balance of protein homeostasis in the liver, causing intracellular protein accumulation due to a disruption of hepatic protein catabolism. Evidence indicates that ethanol or its metabolism impairs trafficking events in the liver, including the process of macroautophagy, which is the engulfment and degradation of cytoplasmic constituents by the lysosomal system. Autophagy is an essential, ongoing cellular process that is highly regulated by nutrients, endocrine factors and signaling pathways. A great number of the genes and gene products that govern the autophagic response have been characterized and the major metabolic and signaling pathways that activate or suppress autophagy have been identified. This review describes the process of autophagy, its regulation and the possible mechanisms by which ethanol disrupts the process of autophagic degradation. The implications of autophagic suppression are discussed in relation to the pathogenesis of alcohol-induced liver injury. 展开更多
关键词 酒精性肝损伤 自噬 蛋白质分解 乙醇代谢 信号通路 基因产物 内分泌因素 可能机制
在线阅读 下载PDF
Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs 被引量:2
17
作者 Ying Peng Zhuo Wang +3 位作者 Xiao-Bo Li Chun-Fu Wu Jing-Yu Yang Yuan Lu 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2009年第18期2220-2227,共8页
AIM:To find a rapid and efficient analysis method of gastrointestinal microflora in Pi-deficient(spleen-deficient) rats and to evaluate traditional Chinese drugs.METHODS:Enterobacterial repetitive intergenic consensus... AIM:To find a rapid and efficient analysis method of gastrointestinal microflora in Pi-deficient(spleen-deficient) rats and to evaluate traditional Chinese drugs.METHODS:Enterobacterial repetitive intergenic consensus-PCR(ERIC-PCR) based assay was performed to examine changes of intestinal microflora in two Pi-deficienct animal models and to evaluate the efficacy of four traditional Chinese drugs as well as a probiotic recipe and another therapy in Pi-deficient rats.RESULTS:A molecular marker was identified for Pi-deficiency in rats.The pharmacodynamic evaluation system,including identified molecular markers(net integral area and abundance of DNA bands),Shannon's index for diversity of intestinal microflora,and Sorenson's pairwise similarity coefficient,was established.The four major clinical recipes of traditional Chinese drugs for Pi-deficiency in rats,especially at their medium dose(equivalence to the clinical dose),produced more pronounced recovery activities in Pi-deficient rats,while higher doses of these recipes did not show a better therapeutic effect but some toxic effects such as perturbation deterioration of intestinal microflora.CONCLUSION:Both fingerprint analysis and identified marker can show Pi-deficiency in rats and its difference after treatment.The identified molecular marker may be applied in screening for the active compounds both in relative traditional Chinese drugs and in pharmacodynamic study of Pi-deficiency in rats. 展开更多
关键词 胃肠道菌群 传统中药 分子标记 大鼠 PCR技术 健脾 治疗效果 活性化合物
在线阅读 下载PDF
Travel of a mis-swallowed long spoon to the jejunum 被引量:1
18
作者 Yan Song Hua Guo Jian-Yong Wu 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2009年第39期4984-4985,共2页
Foreign-body ingestion is a relatively common presentation at emergency departments,but long metallic spoon swallowing is an infrequent occurrence.Unlike most cases of foreign-body ingestion,there have been no reporte... Foreign-body ingestion is a relatively common presentation at emergency departments,but long metallic spoon swallowing is an infrequent occurrence.Unlike most cases of foreign-body ingestion,there have been no reported cases of long foreign bodies reaching the jejunum.We report a rare case of a coffee spoon that was swallowed accidentally and passed through the pylorus and duodenal loop and reached the jejunum,with no complications. 展开更多
关键词 空肠 旅游 紧急情况 十二指肠 异物 并发症 吞食
在线阅读 下载PDF
Morphological study on colonic pathology in ulcerative colitis treated by moxibustion 被引量:8
19
作者 Huan Gan Wu Li Bin Zhou +5 位作者 Da Ren Shi Shi Min Liu Hui Rong Liu Bi Meng Zhang Han Ping Chen Lin Shan Zhang 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 2000年第6期861-865,共5页
AIM To observe the therapeutic effect ofmoxibustion on ulcerative colitis and itsinfluence on the colonic mucosal morphology.METHODS Forty-six patients with ulcerativecolitis were randomly divided into themoxibustion ... AIM To observe the therapeutic effect ofmoxibustion on ulcerative colitis and itsinfluence on the colonic mucosal morphology.METHODS Forty-six patients with ulcerativecolitis were randomly divided into themoxibustion with herbal medicine underneathgroup and the western medicine group.Thirtypatients were treated with the abovemoxibustion and 16 patients with Salicylayefapyridine(SASP).The colonic mucosa of 13patients in the moxibustion group was observedby colonoscopy before and after the treatment.Mucin was also analyzed by H.E and AB-PASstaining.RESULTS Seventeen patients were clinicallycured,12 were improved and 1 unchanged in themoxibustion group.In the control group,5patients were clinically cured,7 improved and 4unchanged.Thirteen patients with active UCwere taken as the subjects for histopathologicanalysis in this study.The colonic mucosallesions were remarkably improved and thecharacteristic of the mucin also changed.Inmost sections,the chronic inflammation ofmucosa was geatly ameliorated(P【0.01).Theinflammatory cell infiltratation much decreased and neutrophils,disapeared in most sections(P【0.001).The goblet cells significantlyincreased(P【0.001);crypt paracrypt abscessor mucosal ulceration was seen(P【0.001).CONCLUSION The rate of cure of ulcerative colitis by moxibustion with herbal medicine beneath is superior to that by SASP. This sort of moxibustion can effectively improve the colonic mucosal lesions and restore the proportion of mucoprotein to near normal. 展开更多
关键词 COLITIS ulcerative/therapy MOXIBUSTION intestinal MUCOSA morphological study
在线阅读 下载PDF
Inflammatory bowel disease:definition,epidemiology,etiologic aspects,andimmunogenetic studies 被引量:2
20
作者 XIA Bing 1, JBA Crusius 2, SGM Meuwissen 2 and AS Pe*ka 2 《世界胃肠病学杂志:英文版》 SCIE CAS CSCD 1998年第5期83-95,共13页
DISEASEDEFINITIONSeveralcausesexistforinflammationofthegutsuchasinfection,toxin,autoimmunereaction,radiation... DISEASEDEFINITIONSeveralcausesexistforinflammationofthegutsuchasinfection,toxin,autoimmunereaction,radiationandischemia.Whenn... 展开更多
关键词 INFLAMMATORY BOWEL disease/epidemiology INFLAMMATORY BOWEL disease/etiology INFLAMMATORY BOWEL disease/genetics INFLAMMATORY BOWEL disease/immunology
在线阅读 下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部